Citrus psorosis and Mirafiori lettuce big-vein ophiovirus coat proteins localize to the cytoplasm and self interact in vivo.
Identifieur interne : 001378 ( Main/Exploration ); précédent : 001377; suivant : 001379Citrus psorosis and Mirafiori lettuce big-vein ophiovirus coat proteins localize to the cytoplasm and self interact in vivo.
Auteurs : Eduardo José Pe A [France] ; Gabriel Robles Luna ; María Cecilia Zanek ; María Belén Borniego ; Carina Andrea Reyes ; Manfred Heinlein ; María Laura GarcíaSource :
- Virus research [ 1872-7492 ] ; 2012.
English descriptors
- KwdEn :
- Amino Acid Motifs, Amino Acid Sequence, Capsid Proteins (chemistry), Capsid Proteins (genetics), Capsid Proteins (metabolism), Citrus (virology), Conserved Sequence, Cytoplasm (metabolism), Gene Expression, Gene Order, Genes, Reporter, Genetic Vectors (genetics), Molecular Sequence Data, Plant Diseases (virology), Plant Leaves (virology), Plant Viruses (genetics), Plant Viruses (metabolism), Plants, Genetically Modified, Protein Binding, Protein Transport, RNA Viruses (genetics), RNA Viruses (metabolism), Sequence Alignment.
- MESH :
- chemical , chemistry : Capsid Proteins.
- chemical , genetics : Capsid Proteins.
- chemical , metabolism : Capsid Proteins.
- genetics : Genetic Vectors, Plant Viruses, RNA Viruses.
- metabolism : Cytoplasm, Plant Viruses, RNA Viruses.
- virology : Citrus, Plant Diseases, Plant Leaves.
- Amino Acid Motifs, Amino Acid Sequence, Conserved Sequence, Gene Expression, Gene Order, Genes, Reporter, Molecular Sequence Data, Plants, Genetically Modified, Protein Binding, Protein Transport, Sequence Alignment.
Abstract
Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.
DOI: 10.1016/j.virusres.2012.08.005
PubMed: 22921760
Affiliations:
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de Strasbourg</orgName></affiliation></author><author><name sortKey="Robles Luna, Gabriel" sort="Robles Luna, Gabriel" uniqKey="Robles Luna G" first="Gabriel" last="Robles Luna">Gabriel Robles Luna</name></author><author><name sortKey="Zanek, Maria Cecilia" sort="Zanek, Maria Cecilia" uniqKey="Zanek M" first="María Cecilia" last="Zanek">María Cecilia Zanek</name></author><author><name sortKey="Borniego, Maria Belen" sort="Borniego, Maria Belen" uniqKey="Borniego M" first="María Belén" last="Borniego">María Belén Borniego</name></author><author><name sortKey="Reyes, Carina Andrea" sort="Reyes, Carina Andrea" uniqKey="Reyes C" first="Carina Andrea" last="Reyes">Carina Andrea Reyes</name></author><author><name sortKey="Heinlein, Manfred" sort="Heinlein, Manfred" uniqKey="Heinlein M" first="Manfred" last="Heinlein">Manfred Heinlein</name></author><author><name sortKey="Garcia, Maria Laura" sort="Garcia, Maria Laura" uniqKey="Garcia M" first="María Laura" last="García">María Laura 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first="Eduardo José" last="Pe A">Eduardo José Pe A</name><affiliation wicri:level="4"><nlm:affiliation>Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357), Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg CEDEX, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Biologie Moléculaire des Plantes du CNRS (UPR 2357), Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg CEDEX</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName><orgName type="university">Université de Strasbourg</orgName></affiliation></author><author><name sortKey="Robles Luna, Gabriel" sort="Robles Luna, Gabriel" uniqKey="Robles Luna G" first="Gabriel" last="Robles Luna">Gabriel Robles Luna</name></author><author><name sortKey="Zanek, Maria Cecilia" sort="Zanek, Maria Cecilia" 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xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Capsid Proteins (chemistry)</term><term>Capsid Proteins (genetics)</term><term>Capsid Proteins (metabolism)</term><term>Citrus (virology)</term><term>Conserved Sequence</term><term>Cytoplasm (metabolism)</term><term>Gene Expression</term><term>Gene Order</term><term>Genes, Reporter</term><term>Genetic Vectors (genetics)</term><term>Molecular Sequence Data</term><term>Plant Diseases (virology)</term><term>Plant Leaves (virology)</term><term>Plant Viruses (genetics)</term><term>Plant Viruses (metabolism)</term><term>Plants, Genetically Modified</term><term>Protein Binding</term><term>Protein Transport</term><term>RNA Viruses (genetics)</term><term>RNA Viruses (metabolism)</term><term>Sequence Alignment</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Genetic Vectors</term><term>Plant Viruses</term><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Cytoplasm</term><term>Plant Viruses</term><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Citrus</term><term>Plant Diseases</term><term>Plant Leaves</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Motifs</term><term>Amino Acid Sequence</term><term>Conserved Sequence</term><term>Gene Expression</term><term>Gene Order</term><term>Genes, Reporter</term><term>Molecular Sequence Data</term><term>Plants, Genetically Modified</term><term>Protein Binding</term><term>Protein Transport</term><term>Sequence Alignment</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrus psorosis (CPsV) and Mirafiori lettuce big-vein virus (MiLBVV) belong to the family Ophioviridae, plant viruses with filamentous nucleocapsids and segmented genomes of negative polarity, causing the worldwide distributed citrus psorosis and lettuce big-vein diseases, respectively. To gain insight into the replication cycle of these viruses, the subcellular localization of the viral coat proteins (CP) was studied. Immunoblot analysis of fractionated extracts derived from natural and experimental infected hosts indicated that the CP of CPsV occurs in the soluble cytoplasmic fraction. The cytoplasmic localization of this protein was confirmed by confocal microscopy of fluorescent protein (FP)-tagged CP following its expression in either CPsV-infected and healthy Citrus sinensis plants or in Nicotiana benthamiana plants. The same localization was observed for FP-tagged CP of MiLBVV. The CPs of CPsV and MiLBBV can undergo homologous and heterologous interactions as revealed by fluorescent lifetime imaging microscopy and co-immunoprecipitation analysis. A putative leucine zipper motif that is conserved among ophiovirus CP sequences may account for these interactions.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Strasbourg</li></settlement><orgName><li>Université de Strasbourg</li></orgName></list><tree><noCountry><name sortKey="Borniego, Maria Belen" sort="Borniego, Maria Belen" uniqKey="Borniego M" first="María Belén" last="Borniego">María Belén Borniego</name><name sortKey="Garcia, Maria Laura" sort="Garcia, Maria Laura" uniqKey="Garcia M" first="María Laura" last="García">María Laura García</name><name sortKey="Heinlein, Manfred" sort="Heinlein, Manfred" uniqKey="Heinlein M" first="Manfred" last="Heinlein">Manfred Heinlein</name><name sortKey="Reyes, Carina Andrea" sort="Reyes, Carina Andrea" uniqKey="Reyes C" first="Carina Andrea" last="Reyes">Carina Andrea Reyes</name><name sortKey="Robles Luna, Gabriel" sort="Robles Luna, Gabriel" uniqKey="Robles Luna G" first="Gabriel" last="Robles Luna">Gabriel Robles Luna</name><name sortKey="Zanek, Maria Cecilia" sort="Zanek, Maria Cecilia" uniqKey="Zanek M" first="María Cecilia" last="Zanek">María Cecilia Zanek</name></noCountry><country name="France"><region name="Grand Est"><name sortKey="Pe A, Eduardo Jose" sort="Pe A, Eduardo Jose" uniqKey="Pe A E" first="Eduardo José" last="Pe A">Eduardo José Pe A</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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